1. Based on the passage, which of the following fragments from Table 1 would migrate the furthest distance from the starting well during electrophoresis?
2. Based on Table 1, what percent of the F1 offspring in Study 1 had white eyes?
3. A chemistry lab measured the pH of a buffer solution after adding different volumes of acid. Use Table 1 to answer the question.
When 6.0 mL of acid was added, the pH was:
4. A lab tested how enzyme concentration affects reaction rate. Reaction rate was measured as product formed per minute under constant temperature and pH. Table 1 lists the results.
Based on Table 1, when enzyme concentration increased from 0.20 mg/mL to 0.40 mg/mL, the reaction rate changed by:
5. Researchers tested how water temperature affects the time needed for a tablet to dissolve in 200 mL of water. Each trial used the same tablet mass and the same stirring rate. The results are shown in Table 1.
According to Table 1, what was the dissolve time when the water temperature was 40 °C?
6. An environmental lab measured nitrate concentration in river water at different distances downstream from a wastewater outlet. Samples were collected the same day and analyzed with the same instrument. Table 1 reports the results.
At a distance of 5.0 km downstream, the nitrate concentration was:
7. A nutrition study compared the caffeine content of several beverages. Each value in Table 1 represents the caffeine measured from a 355 mL (12 oz) serving. The same analytical method was used for all beverages.
Based on Table 1, which beverage had the lowest caffeine content per serving?
8. A microbiology lab incubated bacterial cultures at different temperatures for 12 hours and then measured optical density (OD) at 600 nm as an estimate of cell density. All cultures started with the same initial OD. Table 1 reports the final measurements.
Based on Table 1, at which incubation temperature was the final OD highest?
9. A researcher measured the heart rate of a participant after different exercise intensities on a stationary bike. Each intensity was maintained for 5 minutes, and heart rate was measured immediately afterward. Table 1 shows the results.
Which exercise intensity produced the greatest increase in heart rate compared with rest?
10. Based on Table 2, what is the relationship between blade pitch angle and power output? As the pitch angle increases from 0° to 40°, the power output:
11. Based on Table 2, as the resistance in the circuit increases from 2.0 Ω to 12.0 Ω, the current in the circuit:
12. According to the results of Study 1, as the NaCl concentration increased from 0 mM/kg to 160 mM/kg, the germination rate of *M. sativa* seeds:
13. According to Table 1, how many distinct DNA bands would be visible in Lane 3 of the electrophoresis gel?
14. Based on Study 2, what is the relationship between relative humidity and transpiration rate?
15. Based on Table 1, as the concentration of HCl increases from 1.0 M to 3.0 M, the time required to collect 50 mL of gas:
16. A chemistry class prepared salt solutions with different salt masses dissolved in the same final volume of water (1.00 L). The density of each solution was then measured at 25 °C. Table 1 shows the results.
According to Table 1, what was the density when 100 g of salt was used?
17. A physiology class measured heart rate before and after a 3-minute step test for five students. Use Table 1 to answer the question.
Which student showed the greatest increase in heart rate (post-exercise minus resting)?
18. A materials science team measured the electrical resistance of a wire at different lengths while keeping the wire type and temperature constant. Use Table 1 to answer the question.
According to Table 1, what was the resistance when the wire length was 1.5 m?
19. A food science lab measured the mass loss of apple slices during drying at different oven temperatures. Each slice started with the same initial mass. Use Table 1 to answer the question.
Which temperature resulted in the greatest percent mass loss?
20. A public health team recorded the number of new cases of an illness in five neighborhoods during one week and also measured the percent of residents vaccinated. Table 1 shows the data.
Based on Table 1, which neighborhood had the lowest number of new cases?
21. A plant science experiment tested seed germination under different salt concentrations. After 7 days, students recorded the percent of seeds that germinated and the average root length of the germinated seeds. Use Table 1 to answer the question.
At 0.20 M NaCl, the average root length was:
22. A meteorology club recorded wind speed and air pressure during five afternoon observations. Use Table 1 to answer the question.
Which observation had the highest air pressure?
23. An astronomy class recorded the apparent brightness of a star using a light sensor at different exposure times. 
According to Table 1, what was the sensor reading at an exposure time of 8 s?
24. A geology lab measured the density and porosity of five rock samples. Use Table 1 to answer the question.
According to Table 1, which sample had the lowest density?
25. A public health class compared particulate matter (PM$\_{2.5}$) levels at different locations in a city. Use Table 1 to answer the question.
Which statement about the data in Table 1 is true?
26. An ecology team estimated the abundance of insects in three habitats by counting the number captured in pitfall traps over 24 hours. Use Table 1 to answer the question.
In which habitat was the total number of insects (beetles + ants + spiders) the greatest?
27. A physics lab tested the extension of a spring when different masses were attached. Students recorded the applied force and the spring extension from its resting length. Use Table 1 to answer the question.
Based on Table 1, which load produced the greatest spring extension?
28. A water quality lab measured dissolved oxygen (DO) and water temperature at different depths in a lake. 
According to Table 1, at what depth was dissolved oxygen the lowest?
29. A nutrition study compared the sodium content of several foods per serving.
Based on Table 1, which food has the highest sodium per serving?
30. A lab group measured the boiling point of water at different atmospheric pressures using a pressure chamber.
Which pressure produced the highest boiling point?
31. A biology class tested how light intensity affects photosynthesis in an aquatic plant. For each trial, students measured the rate of oxygen production and the water temperature in the chamber after 10 minutes. Use Table 1 to answer the question.
At a light intensity of 1,500 lx, the oxygen production rate was:
32. An environmental science group measured nitrate concentration in stream water at different distances downstream from a farm. They also recorded water pH at each site. Use Table 1 to answer the question.
Based on Table 1, which sampling site had the lowest nitrate concentration?
33. Suppose the students had conducted a trial in Study 1 using 1.5 M HCl at 20°C. Based on the trend in Table 1, the time required to collect 50 mL of gas would most likely be:
34. Based on the results of Study 1, which of the following best describes the relationship between the mass of the bob and the period of the pendulum?
35. According to Study 2, how does the dissociation of the solute affect the freezing point of the water?
36. According to Table 1, running at 1.0 km/hr causes the metabolic rate of the Lizard to increase by approximately what factor compared to its resting rate?
37. According to the results of Study 1, which launch angle produced the greatest horizontal distance?
38. Based on Study 2, the optimal soil pH for *M. sativa* seed germination among the values tested is closest to:
39. According to Table 1, what was the current measured in the circuit when the voltage was set to 6.0 V?
40. According to Table 1, which of the following oils had the lowest viscosity at 100°C?
41. Suppose the students conducted an additional trial in Study 2 with a spring compression distance of 5.0 cm. The horizontal distance traveled by the ball would most likely be:
42. Suppose the student performed a trial in Study 1 with an incline angle of 25°. Based on the trend in Table 1, the force required would most likely be:
43. According to the results of Study 1, as the mass of the falling object increases, the terminal velocity:
44. Based on Table 1, what was the force required to pull the block when the angle of the incline was 40°?
45. Suppose the students performed an additional trial in Study 1 with the light source placed at 25 cm. Based on the trend in Table 1, the number of bubbles produced would most likely be:
46. According to Study 1, what is the minimum wind speed required for the model turbine to begin generating electrical power?
47. According to the results of Study 1, as light intensity increased from 0 to 800 μmol/m²/s, the transpiration rate:
48. According to the results of Study 1, as the number of moles of NaCl added to the water increases, the boiling point of the solution:
49. According to Table 1, as altitude increases from 0 km to 50 km, atmospheric pressure:
50. A chemistry student measured how reaction temperature affects the time required for a color change to occur. Each trial used the same reactant concentrations. Use Table 1 to answer the question.
At $35,^{\circ}\text{C}$, the reaction time was:
51. A microbiology lab tested bacterial growth at different antibiotic concentrations. After 24 hours, students measured the optical density (OD) of each culture. Use Table 1 to answer the question.
At an antibiotic concentration of 4 µg/mL, the OD$\_{600}$ was:
52. A marine science lab measured salinity and temperature at five ocean sampling stations. 
At Station 3, the salinity was:
53. A lab measured the solubility of a solid in water at different temperatures. Solubility is reported as grams of solute that dissolve in 100 g of water. Table 1 shows the results.
At a temperature of 60 °C, the solubility was:
54. A materials scientist measured electrical resistance of the same wire at different temperatures. The wire length and cross-sectional area were kept constant. Table 1 shows the measured values.
Which statement about the data in Table 1 is true?
55. A botanist measured the average stem growth of seedlings under different light colors for 10 days. All seedlings received the same total light intensity and water each day. Table 1 lists the mean daily growth and final height.
Based on Table 1, which light color produced the highest mean daily growth rate?
56. A geology class measured the mass and volume of several rock samples and calculated density. Table 1 shows the recorded mass, volume, and calculated density.
According to Table 1, which sample had the highest density?
57. A student measured the period of a pendulum for different string lengths. Each period is the average of several swings. Table 1 shows the results.
Which statement about Table 1 is true?
58. An oceanography class measured salinity and dissolved oxygen at different depths at one offshore location. All measurements were taken on the same day. Table 1 shows the results.
According to Table 1, at what depth was dissolved oxygen lowest?
59. A robotics team tested how battery voltage affects motor speed. They measured the motor’s rotational speed after 30 seconds of operation at each voltage setting. 
Comparing Trial 2 and Trial 4, which variable increased?
60. A meteorology station recorded wind speed and air pressure at the same location at different times during one day. Table 1 lists the measurements.
According to Table 1, what was the air pressure when the wind speed was 12 m/s?
61. Suppose the students performed an additional trial in Study 1 with the light source placed at 25 cm. Based on the trend in Table 1, the number of bubbles produced would most likely be:
62. According to Table 1, running at 1.0 km/hr causes the metabolic rate of the Lizard to increase by approximately what factor compared to its resting rate?
63. Suppose the student performed a trial in Study 1 with an incline angle of 25°. Based on the trend in Table 1, the force required would most likely be:
64. Based on Table 1, what was the force required to pull the block when the angle of the incline was 40°?